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KMID : 0364820170530020071
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Korean Journal of Microbiology
2017 Volume.53 No. 2 p.71 ~ p.78
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Rapid bacterial identification using Raman spectrosco
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No Jee-Hyun
Lee Tae-Kwon
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Abstract
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Raman microspectroscopy is a promising tool for microbial analysis at single cell level since it can rapidly measure the
cell materials including lipids, nucleic acids, and proteins by measuring the inelastic scattering of a molecule irradiated by
monochromatic lights. Using Raman spectra provides high specificity and sensitivity in classification of bacteria at the
strain level. In addition, a Raman approach coupled with stabled isotope such as 13C and 2H is able to detect and quantify
general metabolic activity at single cell level. After bacterial detection process by Raman microspectroscopy, interested unculturable cell sorting and single cell genomics can be accomplished by combination with optical tweezer and microfluidic devices. In this review, the characteristics and applications of Raman microspectroscopy were reviewed and summarized in order to provide a better understanding of microbial analysis using Raman spectroscopy.
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KEYWORD
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bacterial detection, cell sorting, Raman microspectroscopy, single cell, stable isotope
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